Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett

By Yvonne A. Barnett

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Genet. 18, 161–172. 49. Gerhard, G. , Phillips, P. , and Cristofalo, V. J. (1991) EGF- and PDGF-stimulated phosphorylation in young and senescent WI-38 cells. Exp. Cell Res. 193, 87–92. 50. Phillips, P. , and Cristofalo, V. J. (1983) 125I-EGF binding activity is stable throughout the replicative life-span of WI-38 cells. J. Cell. Physiol. 114, 311–316. 51. Phillips, P. , Pignolo, R. , and Cristofalo, V. J. (1987) Insulin-like growth factor-I: specific binding to high and low affinity sites and mitogenic action throughout the life span of WI-38 cells.

5 h. Do not shake the bottle because the resultant bubbles may cause irregularities in the final emulsion thickness. 2. In a dark room, the desired amount of emulsion is gently, but thoroughly mixed in a 1:1 ratio with deionized, distilled water. 3. Add 15–20 mL of the 1:1 emulsion/water solution to a container (a slide mailer works well for this) previously set up in a 40°C water bath in the dark. 4. Dip each slide individually into the slide mailer. One dip is sufficient to coat the slide. 5.

Wharton, W. (1984) Newborn human skin fibroblasts senesce in vitro without acquiring adult growth factor requirements. Exp. Cell Res. 154, 310–314. 120. Clemmons, D. R. (1983) Age-dependent production of a competent factor by human fibroblasts. J. Cell. Physiol. 114, 61–67. 121. Kondo, H. and Yonezawa, Y. (1995) Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration. Exp. Cell Res. 220, 501–504. 122. , Reagan, J. , Yan, L.

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