Antibodies - A LABORATORY MANUAL by Ed Harlow and David Lane

By Ed Harlow and David Lane

Show description

Read Online or Download Antibodies - A LABORATORY MANUAL PDF

Best molecular biology books

Molecular Analysis and Genome Discovery

Molecular research and Genome Discovery, moment variation is a totally revised and up to date re-creation of this profitable e-book. The textual content presents a complete evaluate of modern advancements within the fast paced box of molecular dependent diagnostics of affliction markers. Key innovations and purposes are supplied along sensible info on present suggestions presently being researched and built.

Structural Aspects of Protein Synthesis

This hugely illustrated booklet presents an up to date description of the constitution and serve as of the interpretation procedure together with ribosomes, tRNAs, translation elements, antibiotics and aminoacyl-tRNA synthetases. examine on translation is present process quick alterations and is receiving major realization as evidenced via the Nobel Prize in Chemistry 2009.

Stress Responses: Methods and Protocols

This quantity makes a speciality of detecting various mobile stresses, measuring pathological outcomes in the mobilephone, and investigating the position of mobile stresses in opt for ailments. furthermore, this e-book studies the crosstalk among diversified tension pathways, tension responses in the course of getting old, and concentrating on tension for regenerative medication.

Regulation of Ca2+-ATPases,V-ATPases and F-ATPases

The organic membranes of mobile association enfold a big staff of membrane proteins known as the ATPases, which aren't simply flexible in keeping chemical gradient and electric power around the membrane but additionally carry metabolites invaluable for cellphone metabolism and force out pollution, waste items and solutes that differently can shrink phone services.

Additional resources for Antibodies - A LABORATORY MANUAL

Example text

And S. Tonegawa. 1976. Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc. Natl. Acad. Sci. 73: 3628-3632. , N. Hozumi, G. Matthyssens, and R. Schuller. 1977. Somatic changes in the content and context of immunoglobulin genes. Cold Spring Harbor Symp. Quant. Biol. 41:877-889. , M. Hirama, R. Lenhard-Schuller, and S. Tonegawa. 1978. A complete immunoglobulin gene is created by somatic recombination. Cell15: 114. , K. W. L. Livant, R. L. Weissman, and L.

A. G. Laver. 1987. How antibodies recognize virus proteins. lmmunol. Today 8:323- 326. R. 1987. The ant ibody combining site: Retrospect and prospect. lmmunol. Today 8:44-45 ANTIBODY RESPONSE An antibody response is the culmination of a series of interactions between macrophages, T lymphocytes, and B lymphocytes, all reacting to the presence of a foreign antigen. The end product of this response is the production of large numbers of antibody molecules that bind specifically to the antigen and speed its removal from the animal.

The production of a functional heavy chain requires two recombination events, one joining a D region to a J constant region, and another joining a variable region to the D region. There are approximately 50- 100 variable, 12 D, and 4 J chain regions that form the heavy-chain gene cluster on chromosome 12 in the mouse. However, the D regions produce functional proteins in all three reading frames, so, because the recombination events are not precise, the D regions can code for 36 different sequences.

Download PDF sample

Rated 4.48 of 5 – based on 17 votes