Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban

By Lyn Healy, Ludmila Ruban

This lavishly-illustrated, authoritative atlas explores the complex artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide numerous issues in pluripotent stem telephone culturing together with mouse and human fibroblasts, human embryonic stem cells and caused pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in Culture is a complete number of illustrated ideas complemented by means of informative and academic captions interpreting what quality cells appear like and the way they behave in numerous environments. Examples of ideal cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and triggered pluripotent stem mobile colonies. This distinctive and thorough atlas is a useful source for researchers, lecturers, and scholars who're attracted to or operating with stem cellphone culturing.

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19 A comparison between two different cell densities of mouse feeder cells, 3 h after plating. (a, b) Low density. (c, d) High density. Note that the cells have adhered to the culture vessel and are beginning to spread out (a, ×4 magnification; b–d, ×10) 3 Inactivated Mouse and Human Fibroblasts Quality control of feeders should also include an assessment of the ability of a batch of feeders to support the growth and proliferation of undifferentiated PSCs over at least three, preferably five successive passages.

22 (a, b) To the untrained eye, this colony may appear to be a good one, but it is not. The colony shows areas differentiation (black arrows). The areas appearing as white patches of cells are typical of the beginning of the formation of neural rosettes. If left for a further 48 h, the culture would look like the images depicted in Fig. 23. (c) Two colonies with a good, undifferentiated morphology that have merged (all ×10 magnification) 48 a 4 Human Embryonic Stem Cells b c Fig. 23 (a–c) Neural rosettes (white arrows) forming from differentiated areas in the hESC colonies.

As with their murine b counterparts, the cells are uniform and evenly spread, with few apoptotic cells and little floating debris (Both ×4 magnification) 22 a Fig. 6 Human feeders at Day 2 after passaging (a) and Day 4 (b). Again, both cultures are of good quality. The cells are evenly spread, with few apoptotic cells and floating debris. In practice, we would use a Fig. 7 Comparison between two different densities of human fibroblast feeders (MRC-5) 24 h after passaging. Both cultures are of good quality.

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