Biographical Memoirs, Volume 86 by National Academy of Sciences

By National Academy of Sciences

Biographical Memoirs is sequence of essays containing the lifestyles histories and chosen bibliographies of deceased participants of the nationwide Academy of Sciences. The sequence presents a checklist of the lifestyles and paintings of a few of the main exclusive leaders within the sciences, as witnessed and interpreted through their colleagues and friends. They shape a biographical historical past of technology in America--an vital a part of our nation's contribution to the highbrow history of the area.

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This discovery set the direction of Harry’s career for the next 25 years. To prove that the cycle was operating in castor bean endosperm, David Canvin examined the fate of C1- and C2-labeled acetate, and the labeling patterns he obtained were in agreement with the postulated pathways: Succinate produced from acetate served as a precursor for glucose by a reversal of glycolysis. Canvin’s work provided the rigorous “proof of the pudding” that Harry loved! A NEW METABOLIC COMPARTMENT: THE DISCOVERY OF GLYOXYSOMES The next breakthrough came when Bill Breidenbach joined the laboratory as a postdoctoral researcher.

Michael Lord decided to look at the intracellular site of phosphatidyl choline synthesis because this is the major lipid of the endomembrane system of the plant cell. He identified the ER as the site of the final catalytic step in the synthesis of this phospholipid and in the process he developed the magnesium shift technique that has been useful to ER researchers ever since. When two lots of tissues are homogenized in parallel, one in a medium with 1 mM EDTA and the other in a medium with 2 mM magnesium chloride, and when the homogenates are fractionated on parallel sucrose gradients, the ER turns out to have a very different density in HARRY BEEVERS 29 the presence of EDTA, because ribosomes are removed from the ER.

Cell Biol. 44:94-106. 1971 With A. H. C. Huang. Isolation of microbodies from plant tissues. Plant Physiol. 48:637-641. 1972 With J. M. Lord and T. Kagawa. Intracellular distribution of enzymes of the CDP-choline pathway in castor bean endosperm. Proc. Natl. Acad. Sci. U. S. A. 69:2429-2432. 1973 With J. M. Lord, T. Kagawa, and T. S. Moore. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J. Cell. Biol. 57:659-667. 1974 With B. J. Miflin. Isolation of intact plastids from a range of plant tissues.

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