By Rosalyn D. Blumenthal
A state-of-the artwork number of simply reproducible laboratory tools for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in person tumors. Chemosensitivity, quantity 1: In Vitro Assays presents a panel of sixteen in vitro measures of chemosensitivity in adherent and non-adherent cells for unmarried brokers and combos of brokers. as well as immunohistochemical and imaging techniques, those assays contain clonogenic, colorimetric, fluorometric, and physiological assays. Highlights contain picture research to evaluate drug sensitivity, excessive throughput techniques utilizing eco-friendly fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay utilized in biotechnology. A better half quantity, quantity 2: In Vivo types, Imaging, and Molecular Regulators, presents protocols for classifying tumors into reaction different types and customizing chemotherapy regimens to person sufferers.
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Additional info for Chemosensitivity: Volume I: In Vitro Assays (Methods in Molecular Medicine)
Cancer Chemother. Pharmacol. 24, 148–154. 31. , and Santen, R. (1993) Statistical analysis of in vivo tumor growth experiments. Cancer Res. 53, 6042–6050. 32. , and Rustum, Y. (1999) Image analysis for quantitation of solid tumor drug sensitivity. Int. J. Surg. Invest. 1, 133–138. 33. Kubota, K. (2001) From tumor biology to clinical PET: a review of positron emission tomography (PET) in oncology. Ann. Nucl. Med. 15, 471–486. 34. , et al. (2002) Increased uptake of the apoptosis imaging agent (99m)Tc recombinant human Annexin V in human tumors after one course of chemotherapy as a predictor of tumor response and patient prognosis.
Clean a rectangular tub and fill it halfway with ice. Remove the flasks from the incubator and close the caps tightly. Place the flasks on ice, and insert half depth into the ice. Tilt the flasks to the bottom so that the medium does not rest against the cap. Start a timer for 20 min. While waiting, prepare the Coulter counting vials. Label each as 0, 2, 4, and 6 Gy. 9 mL of Isoton (to be used for counting cells if using a Coulter counter) into each vial. Place Isoton in a control vial and run through the counter to get a background measurement; repeat until a satisfactory low background is obtained.
Seeding of Microtiter Plates for Growth Kinetics For cytotoxicity experiments, it is critical to ensure exponential cell growth for the entire duration of the assay. In particular, this is dependent on the number of cells seeded per well in the microtiter plate at the initiation of the experiment. 0. 1. Day 1 1. Harvest cells by trypsinization and determine the cell count according to standard cell culture procedures (see Note 1). 2. , 100–50,000 cells/ well) in microtiter plates in 100 µL of growth medium per well at d 1 (keep one eight-well row as a blank containing only growth medium, usually row 1).